{"id":916,"date":"2021-03-26T06:53:31","date_gmt":"2021-03-26T06:53:31","guid":{"rendered":"https:\/\/www.aquacombine.eu\/?p=916"},"modified":"2021-03-26T07:16:28","modified_gmt":"2021-03-26T07:16:28","slug":"green-fractionation-and-biomass-characterization","status":"publish","type":"post","link":"https:\/\/www.aquacombine.eu\/?p=916","title":{"rendered":"Green fractionation and biomass characterization"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"916\" class=\"elementor elementor-916\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-21d9e44 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"21d9e44\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-190616f\" data-id=\"190616f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-afca992 elementor-widget elementor-widget-heading\" data-id=\"afca992\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Green fractionation and biomass characterization<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-cb505af elementor-section-full_width elementor-section-height-default elementor-section-height-default\" data-id=\"cb505af\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-8013e9c\" data-id=\"8013e9c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f4c7517 elementor-widget elementor-widget-text-editor\" data-id=\"f4c7517\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Work package no. 5 (WP5) focuses on the processing of green and fresh, but not food-grade biomass. Here in Aalborg University (AAU), we have been fractionated and characterized <em>Salicornia ramosissima<\/em>, <em>Salicornia europaea<\/em>, <em>Tripolium pannonicum<\/em> and <em>Crithmum maritimum<\/em> biomasses grown in the greenhouses of RIASEARCH and Leibniz Universit\u00e4t Hannover (LUH).<\/p><figure id=\"attachment_917\" aria-describedby=\"caption-attachment-917\" style=\"width: 300px\" class=\"wp-caption aligncenter\"><img decoding=\"async\" class=\"size-medium wp-image-917\" src=\"https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_1-300x136.jpg\" alt=\"\" width=\"300\" height=\"136\" srcset=\"https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_1-300x136.jpg 300w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_1-685x310.jpg 685w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_1-24x11.jpg 24w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_1-36x16.jpg 36w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_1-48x22.jpg 48w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_1.jpg 743w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><figcaption id=\"caption-attachment-917\" class=\"wp-caption-text\">AQUACOMBINE: Salicornia europaea, Crithmum maritimum and Tripolium pannonicum biomass from LUH.<\/figcaption><\/figure><p>In green fractionation, the plant material is screw pressed to green juice and fibre residue, which are considered individually. The characterization considered the amounts of ash, protein, lipids, organic acids, carbohydrates and lignin in the biomass. We have also studied how the chemical composition changes, if the plant is exposed to different salinities in the cultivation phase. Especially <em>S. europaea<\/em> and <em>T. pannonicum<\/em> showed to be rich in protein and having a low lipid content. As halophytes accumulate salt to their tissues, ash covers a large fraction of the total plant dry matter and the concentration of different minerals were analysed in co-operation with CELABOR. Obtained information about the chemical composition of the considered plants can be used for planning and optimizing the cultivation conditions and biorefinery processes.<\/p><figure id=\"attachment_918\" aria-describedby=\"caption-attachment-918\" style=\"width: 770px\" class=\"wp-caption aligncenter\"><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-918 size-full\" src=\"https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_2.jpg\" alt=\"\" width=\"770\" height=\"565\" srcset=\"https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_2.jpg 770w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_2-300x220.jpg 300w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_2-768x564.jpg 768w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_2-685x503.jpg 685w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_2-24x18.jpg 24w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_2-36x26.jpg 36w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_2-48x35.jpg 48w\" sizes=\"(max-width: 770px) 100vw, 770px\" \/><figcaption id=\"caption-attachment-918\" class=\"wp-caption-text\">AQUACOMBINE: Research is continuing under special conditions. Concentrations of carbohydrates and organic acids in the fresh biomass are determined using HPLC system.<\/figcaption><\/figure><figure id=\"attachment_919\" aria-describedby=\"caption-attachment-919\" style=\"width: 520px\" class=\"wp-caption aligncenter\"><img decoding=\"async\" class=\"wp-image-919 size-full\" src=\"https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_3.jpg\" alt=\"\" width=\"520\" height=\"499\" srcset=\"https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_3.jpg 520w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_3-300x288.jpg 300w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_3-24x24.jpg 24w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_3-36x36.jpg 36w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_3-48x46.jpg 48w\" sizes=\"(max-width: 520px) 100vw, 520px\" \/><figcaption id=\"caption-attachment-919\" class=\"wp-caption-text\">AQUACOMBINE: Weighing Salicornia europaea fibre residue for strong acid hydrolysis, which releases sugar monomers from lignocellulosic biomass.<\/figcaption><\/figure>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-25330b9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"25330b9\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-7915853\" data-id=\"7915853\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-c4281f3 elementor-widget elementor-widget-heading\" data-id=\"c4281f3\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Extraction of protein by acidification<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-c339704 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"c339704\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-74700bc\" data-id=\"74700bc\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-11e4cd4 elementor-widget elementor-widget-text-editor\" data-id=\"11e4cd4\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>AAU is also responsible of WP5 task considering the extraction of protein by acidification. In this task, water-soluble protein is separated from green juice by fermenting it using lactic acid bacteria. Bacteria uses the available sugars in the juice and produces lactic acid, which decreases the juice pH and coagulates protein. This method is developed for grasses and agricultural residues [1], but AQUACOMBINE project is the first one to test it for halophyte biomass.<\/p><p>The high salt concentration of halophyte juice makes it a challenging media for lactic acid bacteria. The ongoing screening study is aiming to find a bacterial strain that can thrive in the saline and acidic conditions and, therefore, be suitable for fermenting the freshly pressed halophyte juice. In the screening, several bacterial strains are tested in different conditions to find the suitable strain for green halophyte biorefinery purposes.<\/p><figure id=\"attachment_920\" aria-describedby=\"caption-attachment-920\" style=\"width: 292px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-920\" src=\"https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_4-292x300.jpg\" alt=\"\" width=\"292\" height=\"300\" srcset=\"https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_4-292x300.jpg 292w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_4-24x24.jpg 24w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_4-36x36.jpg 36w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_4-48x48.jpg 48w, https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_4.jpg 533w\" sizes=\"(max-width: 292px) 100vw, 292px\" \/><figcaption id=\"caption-attachment-920\" class=\"wp-caption-text\">AQUACOMBINE: Preparing samples for HPLC, which analyses the amount of lactic acid in the juice.<\/figcaption><\/figure><p>\u00a0<\/p><ol><li>Kiel, P.; Anderson, M.; L\u00fcbeck, M. A METHOD OF PROVIDING FUNCTIONAL PROTEINS FROM A PLANT MATERIAL 2015.<\/li><\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Green fractionation and biomass characterization Work package no. 5 (WP5) focuses on the processing of green and fresh, but not food-grade biomass. Here in Aalborg University (AAU), we have been fractionated and characterized Salicornia ramosissima, Salicornia europaea, Tripolium pannonicum and Crithmum maritimum biomasses grown in the greenhouses of RIASEARCH and Leibniz Universit\u00e4t Hannover (LUH). In &hellip; <a href=\"https:\/\/www.aquacombine.eu\/?p=916\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">Green fractionation and biomass characterization<\/span><\/a><\/p>\n","protected":false},"author":4,"featured_media":917,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-916","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"jetpack_featured_media_url":"https:\/\/www.aquacombine.eu\/wp-content\/uploads\/2021\/03\/Green-fractionation-and-biomass-characterization_1.jpg","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.aquacombine.eu\/index.php?rest_route=\/wp\/v2\/posts\/916","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.aquacombine.eu\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.aquacombine.eu\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.aquacombine.eu\/index.php?rest_route=\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/www.aquacombine.eu\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=916"}],"version-history":[{"count":3,"href":"https:\/\/www.aquacombine.eu\/index.php?rest_route=\/wp\/v2\/posts\/916\/revisions"}],"predecessor-version":[{"id":944,"href":"https:\/\/www.aquacombine.eu\/index.php?rest_route=\/wp\/v2\/posts\/916\/revisions\/944"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.aquacombine.eu\/index.php?rest_route=\/wp\/v2\/media\/917"}],"wp:attachment":[{"href":"https:\/\/www.aquacombine.eu\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=916"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.aquacombine.eu\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=916"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.aquacombine.eu\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=916"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}